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(1) Restoration of energy homeostasis under oxidative stress: Duo synergistic AMPK pathways regulating arginine kinasesPLoS geneticsAugust 1, 2023Zhang N, Meng X, Jiang H, Ge H, Qian K, Zheng Y, Park Y, Wang J.diammonium citrate (DAC) dissolved in 50:50 water/acetonitrile (v/v). Mass spectra were acquired in a 5800 Plus MALDI TOF/TOF analyzer mass spectrometer (Applied Biosystems, Framingham, MA, USA) in positive

(2) Plasma metabolic fingerprints for large-scale screening and personalized risk stratification of metabolic syndromeCell Reports MedicineJuly 18, 2023Yifan Chen, Wei Xu, Wei Zhang, Renyang Tong, Ancai Yuan, Zheng Li, Huiru Jiang, Liuhua Hu, Lin Huang, Yudian Xu, et al.adding 100nL of matrix slurry and drying for LDI MS analysis. Then, mass spectra were performed on a 5800 Proteomics Analyzer (Applied Biosystems, Framingham, MA, USA) equipped with a Nd:YAG laser (2 kHz, 355

(3) Vibrio MARTX toxin processing and degradation of cellular Rab GTPases by the cytotoxic effector Makes Caterpillars FloppybioRxiv : the preprint server for biologyMay 12, 2023Herrera A, Packer MM, Rosas-Lemus M, Minasov G, Brummel JH, Satchell KJF.System. Western blots were then completed using anti-G/CFP (Invitrogen MA5–15256), anti-GST (Invitrogen A-5800), anti‐HA (Sigma H6908), anti-α-Tubulin (Sigma T6074), anti‐MCF (produced with purified MCF protein

(4) EndophilinA-dependent coupling between activity-induced calcium influx and synaptic autophagy is disrupted by a Parkinson-risk mutationNeuronMay 3, 2023Bademosi AT, Decet M, Kuenen S, Calatayud C, Swerts J, Gallego SF, Schoovaerts N, Karamanou S, Louros N, Martin E, et al.AB_10981827 anti-Dynamin1,Santa Cruz,Cat#: sc-12724; RRID: AB_2230650 anti-Glutathione S-transferase (GST),Invitrogen,Cat#: A5800; RRID: AB_1500200 Alexa Fluor 488 goat anti-mouse IgG1,Invitrogen,Cat#: A21121; RRID: AB_2535764 Alexa

(5) Linagliptin treatment is associated with altered cobalamin (VitB12) homeostasis in mice and humansScientific ReportsJanuary 12, 2023Harald Tammen, Martin Kömhoff, Denis Delić, Søren S. Lund, Berthold Hocher, Sandra Frankenreiter, Rüdiger Hess, Maximilian von Eynatten, Michael Mark, Thomas KleinBiosystems 4700 Proteomics Analyzer) in linear mode, whereas Exp#4 was measured in quadruplicate using a 5800 MALDI-TOF-TOF (Applied Biosystems 5800 Proteomics Analyzer) in reflectron mode. Exp#2 served as a verification

(6) DHX15 is involved in SUGP1-mediated RNA missplicing by mutant SF3B1 in cancerProceedings of the National Academy of SciencesDecember 6, 2022Jian Zhang, Ji Huang, Ke Xu, Peiqi Xing, Yue Huang, Zhaoqi Liu, Liang Tong, James L. Manleyanti-DHX15 (Bethyl Laboratories, A300-389A, 1:1,000), anti-Glutathione S-transferase (GST) (Invitrogen, A5800, 1:1,500), anti-DYKDDDDK (GenScript, A00187, 1:1,000), anti-SUGP1 (Bethyl Laboratories, A304-675A-M,

(7) Origins of glycan selectivity in streptococcal Siglec-like adhesins suggest mechanisms of receptor adaptationNature CommunicationsDecember 1, 2022Barbara A. Bensing et al.performed as described previously5. Anti-GST antibody was used at a 1:500 dilution and was from Invitrogen (A5800). Peroxidase-conjugated goat anti-rabbit IgG was used at a 1:5,000 dilution and was from Sigma (A0545).

(8) A ShK-like Domain from Steinernema carpocapsae with Bioinsecticidal PotentialToxins (Basel)November 2, 2022Jorge Frias, Duarte Toubarro, Gro Elin Kjæreng Bjerga, Pål Puntervoll, João B. Vicente, Rui L. Reis, Nelson Simões(LC-MS grade, Fisher, Lisbon, Portugal). The data were acquired in linear mid-mass positive mode using a 5800 MALDI-TOF/TOF (AB Sciex) mass spectrometer and TOF/TOF Series Explorer Software v.4.1.0 (AB Sciex).

(9) Activity of the nonreceptor tyrosine kinase Ack1 is regulated by tyrosine phosphorylation of its Mig6 homology regionFEBS lettersNovember 1, 2022Kan Y, Miller WT.antibody (clone 4G10) was obtained from MilliporeSigma; rabbit polyclonal anti-GST was from Invitrogen (A-5800, Waltham, MA, USA). Horseradish peroxidase-linked anti-FLAG mouse antibodies were obtained from MilliporeSigma

(10) Hepatic urea, creatinine and uric acid metabolism in dairy cows with divergent milk urea concentrationsScientific ReportsOctober 20, 2022Marie C. Prahl, Carolin B. M. MÃ¼ller, Dirk Albrecht, Franziska Koch, Klaus Wimmers, BjÃ¶rn Kuhlaproteins were digested using trypsin and the molecular masses of the tryptic digest were measured using a 5800 MALDI TOF/TOF Analyzer (Applied Biosystems, Forster City, CA, USA). The spectra were registered in a

(11) Dual control of formin-nucleated actin assembly by the chromatin and ER in mouse oocytesCurrent biology : CBSeptember 26, 2022Wang H, Hu J, Yi K, Ma Z, Song X, Lee Y, Kalab P, Bershadsky AD, Miao Y, Li R.(927–70001, LI-COR) for 30 min at room temperature. Primary antibodies, including rabbit anti-GST (A-5800, Thermo Scientific, 1:2000), mouse anti-His6 (MA1–21315, Thermo Scientific, 1:2000), rabbit anti-RFP

(12) Experimental Investigations on the Structure of Yeast Mitochondrial Pyruvate CarriersMembranesSeptember 22, 2022Li L, Wen M, Run C, Wu B, OuYang B.solution was spotted onto a seed layer spot on the MALDI target. The mass spectrum was collected on a 5800 MALDI-TOF/TOF (Applied Biosystems, Waltham, MA, USA). 2.9. NMR Spectroscopy in DPC Micelles All NMR

(13) Alterations of actin cytoskeleton and arterial protein level in patients with obstructive jaundiceGenetics and Molecular BiologySeptember 12, 2022Hong-Qian Wang, Xiao-Yan Meng, Jin-Min Zhang, Jia-Ying Chen, Bao-Hua Zhang, Fei-Xiang Wuacid (Sigma, St. Louis, MO, USA) in 0.1% trifluoroacetic acid/50% acetonitrile. Mass spectrometry A 5800 MALDI TOF/TOF analyzer (Applied Biosystem, Framingham, MA, USA) was employed for assessments. Mass spectrum

(14) Bro1 binds the Vps20 subunit of ESCRT-III and promotes ESCRT-III regulation by Doa4Traffic (Copenhagen, Denmark)February 1, 2022Buysse D, West M, Leih M, Odorizzi G.membrane, and Western blotting was performed using anti-GST monoclonal antibodies (ThermoFisher, catalog #A-5800, 1:10,000), anti-HA monoclonal antibodies (anti-HA) or rabbit polyclonal antisera raised against Snf7

(15) The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductaseProceedings of the National Academy of SciencesJanuary 25, 2022Delfim Ferreira, Ana C. C. Barbosa, GonÃ§alo P. Oliveira, Teresa Catarino, Sofia S. Venceslau, InÃªs A. C. Pereirathe MALDI plate and allowed to air dry. The data were acquired in Linear Mid Mass Positive mode using a 5800 MALDI-TOF/TOF (AB Sciex) mass spectrometer and TOF/TOF Series Explorer Software v.4.1.0 (AB Sciex).

(16) NMR Detection and Structural Modeling of the Ethylene Receptor LeETR2 from TomatoMembranes (Basel)January 18, 2022Shukun Wei, Yaqing Yang, Yuan Yuan, Lingyu Du, Hongjuan Xue, Bo OuYangsolution was spotted onto a seed layer spot on the MALDI target. The mass spectrum was collected on a 5800 MALDI-TOF/TOF (Applied Biosystems, Waltham, MA, USA). 2.7. NMR Detection for LeETR21–131 Samples were

(17) Deletion of Polyamine Transport Protein PotD Exacerbates Virulence in Glaesserella (Haemophilus) parasuis in the Form of Non-biofilm-generated Bacteria in a Murine Acute Infection ModelVirulenceDecember 31, 2021Ke Dai et al.assisted laser desorption ionization–tandem time of flight mass spectrometry (MALDI-TOF/TOF) using a 5800 Proteomics analyzer (Applied Biosystems) as previously described [Zhang et al., 2016]. Both peptide

(18) Recreational water exposure and waterborne infections in a prospective salivary antibody study at a Lake Michigan beachScientific reportsOctober 15, 2021Egorov AI, Converse R, Griffin SM, Bonasso R, Wickersham L, Klein E, Kobylanski J, Ritter R, Styles JN, Ward H, et al.anti-Cryptosporidium gp15 from Tufts Medical Center, rabbit anti-GST antibody (Invitrogen, Carlsbad, CA, cat. A-5800), and serially diluted control serum samples, with corresponding secondary anti-species antibodies as

(19) Wnt target enhancer regulation by a CDX/TCF transcription factor collective and a novel DNA motifNucleic Acids ResearchSeptember 7, 2021Aravinda-Bharathi Ramakrishnan, Lisheng Chen, Peter E Burby, Ken M Cadigan(Sigma-Aldrich, A8592), Anti-HA (Roche, 11867423001), Anti-His (Cytiva, 27-4710–01), and Anti-GST (Invitrogen, A-5800). Anti-Flag immunoprecipitations were carried out using magnetic beads pre-conjugated with the antibody

(20) An alkali-tolerant phospholipase D from Sphingobacterium thalpophilum 2015: Gene cloning, overproduction and characterizationThe Journal of General and Applied MicrobiologyApril 16, 2021Jing Li, Yiwen Chu, Ruilan Yang, Jiafu Lin, Lin Shao, Li Wangunder 37°C for 30 min. After lyophilization, dissolving with 0.1% formic acid solution and drying on a 5800 target plate, the plate was sent for analysis by the MALDI-TOF/TOF 5800 mass spectrometer (Applied Biosystems

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